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Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-1526 [HIV-1 LTR CAT Reporter Vector (pCD7)] is a deletion mutant where the 5' end of the long terminal repeat (LTR) to position -278 from the transcriptional start site is deleted.To produce this vector clone pC15CAT (BEI Resources ARP-1527) a derivative of pSV0CAT, was cleaved with KpnI, treated with Bal31 exonuclease, blunted and the XbaI linkers ligated. The resultant deletion mutant, pCD7, contains HIV-1IIIB LTR sequences from -278 to +80 located in front of the CAT gene.
The provided bacterial host is Escherichia coli, strain DH5α but strain HB101 is also an acceptable host.
Each vial of ARP-1526 contains 1 mL of ampicillin resistant E. coli, strain DH5α transformed with pCD7. Please refer to the appropriate data sheet for lot-specific information.
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Seikevitz, M., et al. “Activation of the HIV-1 LTR by T Cell Mitogens and the Trans-Activator Protein of HTLV-I.” Science 238 (1987):1575-1578. PubMed: 2825351.
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Seigel, L. J., et al. “Transactivation Induced by Human T-Lymphotropic Virus Type III (HTLV III) Maps to A Viral Sequence Encoding 58 Amino Acids and Lacks Tissue Specificity.” Virology 148 (1986): 226-231. PubMed: 3002031.
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