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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-776 is a human T4-lymphoblastoid cell line initially derived by G. E. Foley and biologically cloned by P. L. Nara.
These cells have been cloned for both poly-L-lysine induced adherence to microtiter plates and viral-induced syncytial/fusigenic sensitivity following infection with either cell-free or cell-associated HIV-1 and HIV-2. Cells are negative for any virus including human retroviruses as determined by electron microscopy and reverse transcriptase analysis. They can be used for virus production, aspects of HIV-1 cell fusion and molecular biology studies and for the analysis of infectivity, antiviral agents and neutralizing antibodies in the assays referenced below.
Growth Characteristics: These cells double approximately every 1 to 2 days and grow as a suspension of single or small (3-to-10 cell) aggregates. The cells are optimally maintained on a rocker platform or roller bottle apparatus and can be split at 1:20 one to two times per week.
Cellular Morphology: Generally a round, individual, slightly refractile cell population that occasionally forms small aggregates as observed under normal culture conditions. Small numbers of individual highly refractile karyocytomegalic cells may also be observed.
Growth Medium: RPMI 1640 supplemented with 10% fetal bovine serum and 1% Penicillin-Streptomycin-Neomycin (Gibco™ 15640055)
ARP-776 is negative for bacteria, Mycoplasma and fungi.
Each vial of ARP-776 contains approximately 4 × 106 cells in RPMI 1640 medium containing 27% fetal bovine serum and 7% dimethyl sulfoxide (DMSO).
Please refer to the attached file(s) for additional information.
Foley, G. E., et al. “Continuous Culture of Human Lymphoblasts from Peripheral Blood of a Child with Acute Leukemia.”Cancer 18 (1965): 522-529. PubMed: 14278051. Nara, P. L., et al. “Simple, Rapid Quantitative, Syncytium-Forming Microassay for the Detection of Human Immunodeficiency Virus Neutralizing Antibody.” AIDS Res. Hum. Retroviruses 3 (1987): 283-302. PubMed: 3481271. Nara, P. L. and P. J. Fischinger. “Quantitative Infectivity Assay for HIV-1 and -2.” Nature 332 (1988): 469-470. PubMed: 3281026.
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