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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. ARP-2090 is a culture of HLM1 cells that are CD4+ and negative for virus particle production, but can be stimulated to produce non-infectious virions. ARP-2090 was generated by transducing HeLa CD4+ cells (ARP-154, contributed by Dr. Richard Axel) with the tat-defective mutant pMtat- (ARP-2085), which contains a termination codon (TGA) in place of the methionine initiator codon (ATG) in the tat gene. The human immunodeficiency virus (HIV) proviral DNA was derived from pHXB2gpt, an infectious molecular clone of HIV-1 IIIB (ARP-398, contributed by Dr. R. Gallo). ARP-2090 cells are negative for virus particle production, but can be induced to express high levels of non-infectious HIV-1 and syncytial cells after transfection or cocultivation with tat-expressing clones, or after stimulation with TNF-α, PMA, or sodium butyrate. A combination of UV light treatment and cocultivation with tat-expressing cells results in the production of infectious virus. Growth Characteristics: ARP-2090 cells can be maintained in culture by adding fresh medium to the adherent cells every 3-4 days. Trypsinization is necessary only once every month or so unless the cells become over-confluent or need to be transferred to an additional flask. Propagation Medium: The recommended propagation medium is DMEM supplemented with 5% fetal bovine serum and 10µg/mL Geneticin.
Tests for bacteria, fungi and mycoplasma were negative. Each vial of ARP-2090 contains approximately 4.4 × 106 cells in 0.6 mL of freeze medium. Post-thaw viability was 94%.
Sadaie, M. R., et al. “Activation oftat-Defective Human Immunodeficiency Virus by Ultraviolet Light.” New Biol. 2 (1990): 479-486. PubMed: 1981148.
Sadaie, M. R. and G. L. Hager. “Induction of Developmentally Programmed Cell Death and Activation of HIV by Sodium Butyrate.” Virology 202 (1994): 513-518.PubMed: 8009866.
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